Deskripsi

Pepsin Antigen Repair Solution

Product Content

Product Description

After cells or tissues are fixed with paraformaldehyde, formalin or other aldehyde reagents, due to the formation of aldehyde bonds and carboxymethyl bonds by antigenic determinants, as well as the cross-linking of protein quality inspection, etc., the spatial structure of proteins is changed, so that the antigenic determinants are closed, and the binding points of antigens and antibodies are reduced, which eventually leads to the relative weakening of the positive detection rate and staining intensity of antigens during immune labeling, showing false negative or low positive rate. The protein crosslinking caused by aldehydes is reversible. Under certain conditions, such as high temperature or protease hydrolysis, the original conformation of the protein can be restored. This process is antigen retrieval. Antigen repair is a key step in the process of immunohistochemistry. There are many kinds of antigen repair methods, and different methods can be selected according to the characteristics of tissue types. Some are suitable for thermal repair, and some are suitable for enzymatic repair. Before the emergence of heat induced antigen repair, trypsin, protease K, pepsin and other enzymes induced antigen epitope repair was the most commonly used method.

This product is pepsin antigen repair solution. The pepsin concentration is 0.4%, and the solvent is dilute hydrochloric acid. The solution is acidic. It can be used to repair interstitial antigen.

Storage

Storage conditions: 4℃ (It is not used for a long time and stored at -20 ℃)

Shipping conditions: Wet ice

Shelf life: 12 months from date of manufacture

Instructions

In the step of immunohistochemistry or immunofluorescence experiment, in the step of antigen repair, pepsin antigen repair drops are directly added to the tissue section to cover the tissue, or the sections are soaked in the repair solution and incubated at 37 ℃ for 10-30 minutes. The specific time depends on the section and repair effect.

Note

1. Restore pepsin antigen repair solution to room temperature before use.

2. In order to obtain the best experimental effect, the best incubation time of antigen repair is suggested to conduct pre-experiment exploration according to different samples and target proteins.

3. Pepsin antigen repair solution is acidic, which may lead to poor tissue morphology on the section. Please pay attention to controlling the incubation time.

4. For your safety and health, please wear experimental clothes and disposable gloves during operation.

For Research Use Only!